TRAM offers advanced genome engineering services using CRISPR-Cas9–mediated targeting in mouse embryonic stem (ES) cells, enabling the generation of genome engineered mouse models for a wide range of research applications. This approach allows the precise modification of even large and complex genomic regions that are often difficult to target using standard zygote editing techniques.

Our ES cells targeting services include:

Targeting Vector Design

Comprehensive design of targeting vectors, including bioinformatic analysis and cloning services. For challenging genomic regions, we provide optimized alternative cloning and targeting solutions.

ES Cell Electroporation and Screening

Electroporation, expansion, and cryopreservation of individual ES cell colonies. Positive targeting events are identified using PCR and Southern blot analysis.

Blastocyst Injection and Chimeric Mice Production

Injection of positively targeted ES cells into B6D2F1 hybrid or tetraploid blastocysts, producing either high-grade chimeras or fully ES cell–derived animals.