At TRAM, we offer a complete package of services covering every stage of targeted mouse production — from project design to the validation of genetically modified lines. Our advanced technologies and experienced team ensure high precision and efficiency, helping researchers achieve their goals with minimal time and resource expenditure. Our services include:

Targeting Strategy and DNA Template Design

We offer full bioinformatic analysis, vector design, and cloning of targeting constructs. Our team also develops and validates Southern blot strategies, including probe generation, and designs customized TaqMan qPCR assays tailored to each project.

CRISPR/Cas9 Component Design and Testing

We design and perform specific mouse line in vivo validation of target-specific CRISPR/Cas9 components to ensure highest on-target efficiency.

High Efficiency Microinjection Services 

Using our innovative microinjection technology, CRISPR/Cas9 reagents and DNA templates are delivered directly into the cytoplasm of B6D2F1 zygotes, ensuring exceptional survival rates and optimal editing efficiency.

Comprehensive Genotyping and Validation

We perform TaqMan qPCR analysis of integration sites in F0 and F1 offspring, complemented by sequencing and Southern blot verification. All F1 and F2 progeny are thoroughly analyzed to confirm accurate integration and exclude genomic rearrangements.