We perform TaqMan qPCR analysis of integration sites in F0 and F1 offspring, complemented by sequencing and Southern blot verification. All F1 and F2 progeny are thoroughly analyzed to confirm accurate integration and exclude genomic rearrangements.

Download Custom genotyping order form

Development of High-Efficiency qPCR Genotyping Assays

TRAM designs and validates custom TaqMan qPCR genotyping assays to ensure precise, fast, reliable, and high-throughput identification of genetically modified allelessignificantly reducing time and cost in routine genotyping workflows. In addition, TRAM offers a comprehensive genotyping service, including assay design, genomic DNA isolation, and complete qPCR analysis — providing researchers with accurate and ready-to-use results for efficient colony management.