TRAM offers the generation of embryonic stem (ES) cells from already established mouse lines to support a wide range of research including organoid generation which could be used in clinical and translational research.

By using ES cell based systems, researchers can significantly reduce the number of live animals required in downstream experiments, fully supporting the 3R principles of Replacement, Reduction and Refinement while maintaining scientific excellence and ethical responsibility.

Generation of CRISPR/Cas9-Targeted ES Cell Lines for Organoid Production

TRAM offers the generation of CRISPR/Cas9-targeted embryonic stem (ES) cell lines specifically tailored for organoid research and functional studies. Using precise genome-editing strategies, we introduce defined genetic modifications into ES cells, providing a powerful and flexible platform for modeling development, disease mechanisms, and therapeutic interventions in vitro. Each ES line is carefully characterized for template integration, pluripotency, genomic integrity, and targeting accuracy.

By enabling the study of complex biological processes in organoid systems rather than live animals, this service directly contributes to the 3R principles - Replacement, Reduction, and Refinement.