Mice share key physiological and genetic similarities with humans, making them indispensable for modeling human diseases. Advances in transgenic technologies have enabled the integration of human or modified genes into the mouse germ line, allowing researchers to investigate disease mechanisms and test therapeutic strategies in vivo. A major breakthrough in this field is gene knockout and conditional targeting technology, which allows precise modification of endogenous genes and their transmission through generations, greatly expanding the relevance and versatility of mouse models in biomedical research.

Since 1996, TRAM has served as a centralized core facility dedicated to generating and supporting genetically modified mouse models. The unit specializes in the production of transgenic lines, gene-targeted (conventional, knock-in, and conditional) models, and chromosome-engineered strains. In addition, TRAM provides comprehensive support to research groups, including construct design, cloning, DNA recombineering, embryo and sperm cryopreservation, and strain re-derivation. These services help to reduce research costs, minimize animal use, and accelerate scientific discovery through precision, innovation, and efficiency.

The TRAM’s innovations in embryo microinjection and breeding management represent significant progress in implementing the Reduction and Refinement principles of the 3Rs. Its patented electro-impulse microinjection technology markedly improves embryo survival and developmental rates, enabling highly efficient CRISPR-Cas9 delivery while reducing the number of required zygotes and donor animals by up to 80%. Equally important, the integration of precise estrus cycle detection using the MED-pro100 device refines breeding strategies by ensuring that mating occurs only during optimal stage and avoiding use ofanimals in anestrus stage. This minimizes unsuccessful pairings, reduces stress and discomfort in female mice, and enhances overall reproductive efficiency and welfare. Together, these advancements substantially decrease animal use, improve experimental reliability, and promote higher ethical standards in biomedical research.

The unit is fully equipped for 

1) Molecular biology procedures such as cloning, mutagenesis etc. 2) Cell culture. 3) Microinjection with 2 complete setups for pronuclear, intracytoplasmic, and blastocyst injections. The animal house is equipped with individually ventilated cages and is operated under specific pathogen free (SPF) conditions. Mice can be housed under sterile conditions until they are transferred to the care of the customer.