Lab course for the generation of 2D-gels. Bring your own samples. The technique of differential gel electrophoresis will be explained. 1-3 persons, 3-4 days.
Lab course for the identification of pre-separated proteins. Your samples can be prepared here (PAGE), or you bring a gel spot or fractions. 3-5 days. 1-3 persons
Lectures and hands-on training for the general techniques of proteome analysis (2D-PAGE, chromatography, mass spectrometry).
Protein assignments based on peptide maps need to be carefully evaluated due to the statistical character of database searches. Modifications, isoforms and the presence of multiple proteins may influence the results. A practical training to understand the data. 1 day, 1-3 persons