Volume 1, #3, 2007

NOVA

REVIEWS

Mass spectrometry applications in virologyAlexander Hahn and Frank Neipelpp 161-169Abstract:Mass spectrometry has found a growing number of applications in many disciplines of the life sciences. This review will focus on the field of molecular and clinical virology. Examples for established as well as potential applications for mass spectrometry techniques will be given from different fields of virology, among them receptor identification, identification of cellular interaction partners, post translational modification analysis, virus particle structure and clinical applications.Proteomics of Nocardia africana (SD769) recently isolated from patients with pulmonary infection in SudanMogahid M. El Hassan, Kanury V.S. Rao, Zaved Siddique, Dinesh S. Kumar, Rashmi Tickoo, Nageeb S. Saeed, Moawia M. Mukhtar and Mohamed E. Hamidpp 171-177Abstract:Nocardia africana is a pulmonary pathogen which has been recently isolated from human in Sudan. There is great need for proteomic investigations but they are sparse at present. First attempts on N. africana SD 769 (DSM44491) visualized 74 protein of molecular weights between 10 -250 kDa and isoelectric points between 4–7. Due to the limited database information, identified proteins were assigned by homology to sequences from the other bacterial species such as Staphylococcus aureus, Oceanobacillus iheyensis, N. farcinica and Yersinia pestis.

PAPER

Protein kinase B autophosphorylation affects activityMartin Zeller, Bodo Levkau and Simone Königpp 179-186Abstract:Protein kinase B (PKB) plays an important role in various signal transduction pathways. PKB in its active state is phosphorylated on Thr308 and Ser 473 and phosphorylates various target proteins. Mass spectrometric data suggests that PKB also has a high potential for autophosphorylation at random sites on expense of regulatory sites affecting protein activity.

APPLICATION NOTE

Mass spectrometric monitoring of membrane processing: Neuropeptide digestion by skin cellsSimone König and Thomas E. Scholzenpp 187-191Abstract:A method is described which allows the generation of bioactive endothelial cell membrane preparations for subsequent processing of proopiomelanocortin peptides. Semi-quantitative mass spectrometry is used as a detector for monitoring of the products of membrane protease (neutral endopeptidase and angiotensin-converting enzyme) action.

PROCEEDINGS OF THE 3RD MÜNSTER CONFERENCE ON SINGLE CELL ANALYSIS 2006

pp 193-215INTRODUCTIONProteomics, genomics, cytomics...Simone Königpp 193-194SPEAKER ABSTRACTSpp 194-208Techniques for probing the brain`s chemistry one cell at a timeJonathan V. SweedlerFire and ice - First results from the space mission StardustThomas StephanCytomics and the resolution of organismal biocomplexityGünter K. ValetCombined cell sorting and few cell gene expression profiling for the characterisation of GABAergic interneurons and neural precursorsAndreas BosioSingle object tracking as tool to analyze virus entry and cell biological transport phenomenonsMario Schelhass, H. Ewers, I. Sbalzarini, P. Day, J. Schiller, and A. HeleniusAquaporin 2 activity in renal epithelial cells monitored with digital holographic microscopyJens Klokkers, Patrik Langehanenberg, Bayram Edemir, Björn Kemper, Gert von Bally, and Eberhard SchlatterFollowing primary hyperparathyroidism from surgery to proteomic analysisPayam Minofaar, Martin Eisenacher, Dave S. B. Hoon, Christian Stephan, and Frederick R. SingerCombined transcriptome, proteome, and metabolome analysis at a cell type-specific levelJulia KehrMultiplex miRNA profiling from single embryonic stem cells and early embryosRalph VogelsangIntraneuronal crystallin beta2 (crybb2)triggers elongation of axonsThomas Liedke, Uwe Schroer, Jens Christian Schwamborn, and Solon ThanosProteins in action: Monitored by tr (time-resolved) FTIR spectroscopyKlaus GerwertSingle cell detection by time-of-flight secondary ion mass spectrometryDaniel Breitenstein, E. Tallarek, M. Fartmann, R. kersting, and B. HagenhoffMultiplex RT-PCR of DNA modification genes in single blastomeres of mouse preimplantation embryosAndreas May, Ulrich Zechner, and Thomas HaafNon-contact microdissection for pure DNA, RNA, proteins and live cellsRenate BurgemeisterMolecular profiling of herbal and animal tissue by direct infrared laser desorption ionization mass spectrometryKlaus DreisewerdFrom mass imaging to class imaging - New Horizons in MALDI molecular imagingSören-Oliver Deininger, Arnd Ingendoh, Martin SchürenbergAdvances in tissue imaging using a MALDI quadrupole oa-TOF mass spectrometerRonan O`Malley, Marten Snel, Daniel Kenny, Emmanuelle Claude, Richard Tyldesley-Worster, and James LangridgeImaging of proteins and small molecules using the 4800 MALDI-TOF/TOF analyzerAndrew James, Matthew Willetts, Matthias Glückmann, Volker Kruft

POSTER ABSTRACTS

pp 208-215Application of nanotechnologies for analyzing virulence factors of pathogenic bacteria: Shiga toxin 1-caused damage of human brain microvascular endothelial cellsAndreas Bauwens, Martina Bielaszewska, Helge Karch, Henning Schweppe, Alexander Friedrich, Rudolf Reichelt, Björn Kemper, Patrik Langenhanenberg, Gert von Bally, Jana Hüve, Martin Kahms, Reiner Peters, Michael Mormann, Jasna Peter-Katalinic, and Johannes MüthingNative UV laser induced fluorescence detection for single cell analysis in microfluidic devicesDominik Greif, Wibke Hellmich, Dario Anselmetti, and Alexandra RosSingle-cell multi-analysis (FISH, IHC, single cell CGH) of early flat urothelial lesionsStella Koufou, S. Langer, M. Speicher, and R. KnüchelImmune modulators expressed in laser microdissected keratinocytes during the early phase of experimental leishmaniasis and the potential role of I TAC for Th2-cell differentiationKirsten Roebrock, J.M. Ehrchen, D. Föll, H. J. Schuberth, and J. RothNeprylisin and angiotensin-converting enzyme: Peripheral control of proopiomelanocortin peptide bioactivity by endothelial cell-derived peptidasesThomas E. Scholzen, Thomas A. Luger, and Simone KönigHepatic targeting using transplantation of single cellsAndree Zibert, Jörg Haberland, and H. H.-J. Schmidt