Analysis of synaptic function using electrophysiological procedures

To complement our molecular genetic, biochemical and morphological studies we perform electrophysiological recordings. Using mostly the whole-cell patch-clamp technique we record from nerve cells of genetically modified mouse models, and compare several aspects of their neurotransmission. The focus of our analyses is on basic transmission, spontanuous vesicle release, and common forms of synaptic short-term plasticity.

In addition, we perform recordings on heterologous cell lines stably or transiently tranfected with fluorescently tagged fusion proteins as well as on transfected cells in organotypic cultures.