Nicht-invasive Bildgebung, Zell-Tracking und funktionelle Analysen an zellulären Barrieren

    Forschungsgebiet: Kleintier-MRI, subzelluläre Bildgebung

    Prof. Dr. rer. nat. Cornelius Faber
    Prof. Dr. Johannes Roth (seit 07/2016)
    Prof. Dr. Karin Loser (seit 07/2016)

    Projektlaufzeit: Juli 2012 - Juni 2020

    • NDP-MSH inhibits the progression of ongoing EAE. MRI of brains from NDPMSH–treated mice and controls at disease maximum. Two representative images of a multiplanar reconstruction per group are shown. Signal enhancement in the brain due to contrast agent accumulation is marked by white arrows. - © C. Faber / K. Loser

      Dieses zentrale Projekt bietet dem SFB alle erforderlichen Geräte, Methoden und Protokolle für:

      1. ex vivo Analysen von Zellen in zellulären sowie Tiermodellen, unter der Verwendung von State-of-the-art Methoden des Flow Cytometry Imaging;
      2. nicht-invasive in vivo Bildgebung mittels MRT.

      Neue MRT Methoden werden entsprechend der Anforderungen der einzelnen Teilprojekte entwickelt für die Detektion einzelner Zellen, die ultra-hoch aufgelöste Darstellung von Zellmigration in der Haut, die Detektion infiltrierender Immunzellen in der Lunge oder die simultane Darstellung von Immunzellen und Pathogenen.

    • Publikationen

      Originalartikel

      • Freise N, Burghard A, Ortkras T, Daber N, Chasan AI, Jauch S, Fehler O, Hillebrand J, Schakaki M, Rojas J, Grimbacher B, Vogl T, Hoffmeier A, Martens S, Roth J, Austermann J (2019) Regulatory signaling mechanisms of phagocyte re-programming during systemic vascular inflammation. Blood 134:134-146.
      • Masthoff M, Buchholz R, Beuker A, Wachsmuth L, Kraupner A, Albers F, Freppon F, Helfen A, Gerwing M, Höltke C, Hanson U, Rehkämper J, Vielhaber T, Heindel W, Eisenblaetter M, Karst U, Wildgruber M, Faber C (2019) Introducing specificity to iron oxide nanoparticle imaging by combining 57Fe-based MRI and mass spectrometry. Nano Lett 19(11):7908-7917.
      • Breuer J, Loser K, Mykicki N, Wiendl H, Schwab N. Does the environment influence multiple sclerosis pathogenesis via UVB light and/or induction of vitamin D? J Neuroimmunol. 2018 May 18. pii: S0165-5728(17)30478-2.
      • Masthoff M, Gran S, Zhang X, Wachsmuth L, Bietenbeck M, Helfen A, Heindel W, Sorokin S, Roth J, Eisenblätter M, Wildgruber M, Faber C (2018). Temporal window for detection of inflammatory disease using dynamic cell tracking with timelapse MRI, Sci Rep 8:9563
      • Breuer, J, Herich S, Schneider-Hohendorf T, Chasan AI, Wettschureck N, Gross CC, Loser K, Zarbock A, Roth J, Klotz L, Wiendl H, and Schwab N. (2017) Dual-action by dimethyl fumarate synergistically reduces adhesion to human endothelium. Multiple Scler. J. 1352458517735189.
      • Vogl T, Stratis A, Wixler V, Völler T, Thurainayagam S, Jorch SK, Zenker S, Dreiling A, Chakraborty D, Fröhling M, Paruzel P, Wehmeyer C, Hermann S, Papantonopoulou O, Geyer C, Loser K, Schäfers M, Ludwig S, Stoll M, Leanderson T, Schultze JL, König S, Pap T, Roth J. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest. 2018 May 1;128(5):1852-1866. doi: 10.1172/JCI89867.
      • Wegner J, Loser K, Apsite G, Nischt R, Eckes B, Krieg T, Werner S, Sorokin L (2016) Laminin alpha5 in the keratinocyte basement membrane is required for epidermal-dermal intercommunication. Matrix Biol. 56:24-41.
      • Klasen T, Faber C (2016). Assessment of the myelin water fraction in rodent spinal cord using T2-prepared ultrashort echo time MRI. Magn Reson Mater Phy, 1-10.
      • Mykicki N, Herrmann AM, Schwab N, Deenen R, Sparwasser T, Limmer A, Wachsmuth L, Klotz L, Köhrer K, Faber C, Wiendl H, Luger TA, Meuth SG, Loser K (2016). Melanocortin-1 receptor activation is neuroprotective in mouse models of neuroinflammatory disease. Sci Transl Med, 8 (362): 362ra146.
      • O'Halloran PJ, Viel T, Murray DW, Wachsmuth L, Schwegmann K, Wagner S, Kopka K, Jarzabek MA, Dicker P, Hermann S, Faber C, Klasen T, Schäfers M, O'Brien D, Prehn J HM, Jacobs AH, Byrnel AT (2016). Machanistic interrogation of combination Bevacizumab/dual P13K/m TOR inhibitor response in Glioblastoma implementing novel MR and PET imaging biomarkers. Eur J Nucl Med Mol Imaging, 43(9): 1673-1683.
      • Song J, Wu C, Agrawal S, Korpos E, Wang Y, Faber C, Schäfers M, Körner H, Opdenakker G, Hallmann R, Sorokin L (2015) Focal MMP-2 and MMP-9 activity at the blood-brain barrier (BBB) promotes chemokine-induced leukocyte migration. Cell Reports 10 (7): 1040-1054.[doi: 10.1016/j.celrep.2015.01.037] Epub 2015 Feb 19.
      • Hoerr V, Faber C (2014) Magnetic resonance imaging characterization of microbial infections. J Pharm Biomed Anal 93: 136-146.
      • Schmidt R, Nippe N, Strobel K, Masthoff M, Reifschneider O, Castelli DD, Höltke C, Aime S, Karst U, Sunderkötter C, Bremer C, Faber C (2014) Highly Shifted Proton MRI - Cell tracking by direct detction of paramagnetic compounds. Radiology 272(3): 785-795. (doi: 10.1148/radiol.14132056. Epub 2014 May 18)
      • Hoerr V, Tuchscherr L, Hüve J, Nippe N, Loser K, Glyvuk N, Tsytsyura Y, Holtkamp M, Sunderkötter C, Karst U, Klingauf J, Peters G, Löffler B, Faber C (2013) Bacteria tracking by in vivo magnetic resonance imaging. BMC Biol 11: 63. [doi: 10.1186/1741-7007-11-63]