Epigenetic basis of the plasticity properties and therapy resistance of T-line derived myeloid leukemia stem cells

The project focuses on leukemia cells deriving from t-lymphocyte precursors, characterized by their unique lineage plasticity. Originally displaying both myeloid as well as t-lymphocyte marker, some cells possess the ability to switch their lineage from a myeloid signature to a t-lymphocyte phenotype. In human patients, lineage instability is often accompanied by therapy resistance and a negative prognosis. Our aim is to find subpopulations within the leukemic cell population leading to different lineage plasticity behavior via single cell RNA Sequencing and tackling epigenetic mechanisms controlling the lineage switch with ATAC-Seq (Assay for Transposase-Accessible Chromatin + Sequencing).

Project number: 70112660