Welcome to the IZKF Core Unit for Stem Cell Technologies (SCT). We provide the infrastructure, technical expertise, and standardized protocols necessary to make advanced stem cell technologies accessible to the broader research community.

Induced pluripotent stem cells (iPSCs) have become an indispensable tool in modern medical research. By reprogramming somatic cells, researchers can access a cellular model system that:

• Self-renews indefinitely, providing a consistent and scalable resource.
• Differentiates into virtually any human cell type, enabling the study of diverse tissues in vitro.
• Captures donor-specific genetics, allowing for the modeling of disease mechanisms within a relevant human genetic background.

Over the last 15 years, the field has evolved from simple 2D cultures to 3D organoid models. These models allow for the generation of complex, multicellular environments that closely mimic the physiological niches found in the human body, offering a powerful alternative to traditional 2D cultures and animal models.

The primary goal of the SCT Core Unit is to lower the entry barrier for iPSC-based research at the Faculty of Medicine. We offer an internationally competitive service portfolio, ensuring that researchers can integrate high-quality stem cell models into their projects without the need for specialized in-house infrastructure.

• Reprogramming: Generation of iPSCs from fibroblasts and PBMCs.
• Genome Editing: Precision engineering, including knockouts and point mutations.
• Differentiation: Specialized protocols for 2D cell types and 3D organoid models.
• Consultation & Training: Guidance on project design, experimental setup, and technical training.
• iPSC Library: We are currently building a shared library of characterized iPSC lines for communal use.

To discuss a potential project, please download our Project Intake Form and contact our team at: cusct@uni-muenster.de.

Coordination Team:

• Prof. Michael Ziller (ziller@uni-muenster.de)
• Prof. Tanja Kuhlmann (tanja.kuhlmann@ukmuenster.de)
• Dr. Yotam Menuchin-Lasowski (Yotam.menuchin-lasowski@ukmuenster.de)

The following services will be available soon:

• iPSC Line Reprogramming
• Genome Editing – Knockout
• Genome Editing – Point Mutation
• QC – Digital Karyotyping
• QC – 3-Lineage Differentiation
• Purchase of Existing iPSC Line
• 2D Culture Differentiation
• Cortical Organoids
• Midbrain Organoids
• Thalamic Organoids
• Retinal Organoids

Coming Soon: We are currently establishing protocols for Kidney Organoid and Bone Marrow Organoid generation. These services will be available in the near future.