Dr. Christian Schuberth
Medizinische Fakultät der Westfälische Wilhelms-Universität
Von-Esmarch-Straße 56, Gebäude 2352, Raum 130.101Tel.: +49 251 83-59053
Fax: +49 251 83-59060Email: cschuber@uni-muenster.de
I am interested in studying basic properties of single eukaryotic cells. In particular, my research focus lies on the dynamics of the cytoskeleton and its interconnection with cellular membranes (plasma membrane, Golgi and endoplasmatic reticulum). Currently, I am involved in the following projects:
1) Inf2 mediated actin reorganization in mammalian cells: CaAR ("Calcium-mediated Actin Reset")
2) Dynamic (self-)organization of proteins and actin cables at the yeast cortex
The main methods I use are:
- Genetic manipulation of yeast and mammalian cells (genome editing, gene silencing, protein tagging)
- Advanced fluorescence microscopy techniques (TIRF, FRAP, Laser ablation)
- Quantitative image analysis
Publications
Mietkowska M, Schuberth C, Wedlich-Söldner R, Gerke V (2019). Actin dynamics during C2+-dependent exocytosis of endothelial Weibel-Palade bodies. Biochim. Biophys. Acta Mol. Cell Res. 1866(7): 1218-1229. doi: 10.1016/j.bbamcr.2018.11.010
Granado D, Müller D, Krausel V, Kruzel-Davila E, Schuberth C, Eschborn M, Wedlich-Söldner R, Skorecki K, Pavenstädt H, Michgehl U, Weide T (2017). Intracellular APOL1 Risk Variants Cause Cytotoxicity Accompanied by Energy Depletion. J. Am. Soc. Nephrol. 28(11): 3227-3238. doi: 10.1681/ASN.2016111220
Chehab T**, Santos NC**, Holthenrich A, Koerdt SN, Disse J, Schuberth C, Nazmi AR, Neeft M, Koch H, Man KNM, Wojcik SM, Martin TFJ, van der Sluijs P, Brose N, Gerke V (2017). A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells. Mol. Biol. Cell 28(12):1688-1700. doi: 10.1091/mbc.E17-02-0128
Wales P**, Schuberth CE**, Aufschnaiter R**, Fels J, García-Aguilar I, Janning A, Dlugos CP, Schäfer-Herte M, Klingner C, Wälte M, Kuhlmann J, Menis K, Hockaday Kang L, Maier KC, Hou W, Russo A, Higgs HN, Pavenstädt H, Vogl T, Roth J, Qualmann B, Kessels MM, Martin DE, Mulder B, Wedlich-Söldner R (2016). Calcium-mediated actin reset (CaAR) mediates acute cell adaptions. Elife 2016 Dec 6;5. pii: e19850. doi: 10.7554/eLife.19850
Schuberth C, Wedlich-Söldner R (2015). Building a patchwork - The yeast plasma membrane as model to study lateral domain formation. Biochim. Biophys. Acta 1853(4):767-74. doi: 10.1016/j.bbamcr.2014.12.019 Review
Schuberth CE*, Tängemo C, Tischer C, Coneva C and Pepperkok R (2015). Self-organization of core Golgi material is independent of COPII-mediated endoplasmic reticulum export. J. Cell Sci. 128(7): 1279-93. doi: 10.1242/jcs.154443
Thormaehlen AS, Schuberth C, Won H-H, Blattmann P, Joggerst-Thomalla B, Theiss S, Asselta R, Duga P, Merlini PA, Ardissino D, Lander ES, Gabriel S, Rader DJ, Peloso G, Pepperkok R, Kathiresan S and Runz H (2015). Systematic cell-based phenotyping of missense alleles empowers rare variant association studies: a case for LDLR and myocardial infarction. PloS Genet. 11(2): e1004855. doi:10.1371/journal.pgen.1004855
Medina MW, Bauzon F, Naidoo D, Theusch E, Stevens K, Schilde J, Schubert C, Mangravite LM, Rudel LL, Temel RE, Runz H and Krauss RM (2014) Transmembrane protein 55B is a novel regulator of cellular cholesterol metabolism. Arterioscler. Thromb. Vasc. Biol. 34(9):1917-23. doi: 10.1161/ATVBAHA.113.302806
Blattmann P, Schuberth C, Pepperkok R and Runz H (2013). RNAi-Based Functional Profiling of Loci from Blood Lipid Genome-Wide Association Studies Identifies Genes with Cholesterol-Regulatory Function. PloS Genet. 9 (2): e1003338. doi: 10.1371/journal.pgen.1003338
Schuberth C* and Buchberger A (2008). UBX domain proteins: major regulators of the AAA ATPase Cdc48/p97. Cell. Mol. Life Sci. 65(15): 2360-2371. doi: 10.1007/s00018-008-8072-8 Review
Schuberth C and Buchberger A (2005). Membrane-bound Ubx2 recruits Cdc48 to ubiquitin ligases and their substrates to ensure efficient ER-associated protein degradation. Nat. Cell Biol. 7(10): 999-1006.
Schuchardt I, Assmann D, Thines E, Schuberth C and Steinberg G (2005). Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Long-Distance Transport in Hyphal Growth of the Fungus Ustilago maydis. Mol. Biol. Cell. 16(11): 5191-5201.
Schuberth C, Richly H, Rumpf S and Buchberger A (2004). Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation. EMBO Rep. 5(8): 818‑24.
* corresponding author; ** equal contribution