Current research area
  • Regulation of organic cation transporters and interaction with intracellular proteins
  • Role of  organic cation transporters in cellular toxicities of various drugs in rat, mouse and man
  • Function of organic cation transporters in various diseases
  • Analyses of acute rejection after kidney transplantation with modern imaging

Current techniques

  • Isolation of single nephron segments of rat, mouse and man
  • Generation of primary cultures of renal tubular cells of rat and mouse
  • Culture of renal tubular cell lines of murine, porcine, canine, and human origin
  • Transient and stable gene expression in HEK293-cells
  • Generation and expression of mutants in HEK293-cells
  • Split ubiquitin yeast two hybrid analysis of protein interactions
  • Microfluorimetrical analysis of intracellular ion concentrations
  • Microfluorimetrical analysis of organic cation transport via the fluorescent cation 4-di-1-ASP
  • Quantitative and semi quantitative RT-PCR, and Western-blot analyses 
  • Immuno-histochemistry, immuno-fluorescence
  • Experimental kidney transplantation models in rats with and without acute and chronic rejection and immuno-suppression
  • Ischemia/reperfusion models in rats and mice