CV & Publications

 

Prof. Dr. Klaus Ebnet
Group Leader Institute-Associated Research Group "Cell Adhesion and Cell Polarity"
Institute of Medical Biochemistry
ZMBE

News

 

We have edited a book on Cell Polarity.

The book comes in 2 volumes:

 

Volume 1(Biological Role and Basic Mechanisms) describes the molecular toolkit that is used to generate cell polarity and how this limited set of polarity molecules regulates various aspects of cell polarity in different cell types.

 

Volume 2(Role in Development and Disease) puts a major emphasis on the physiological role of cell polarity and describes the role of cell polarity during development and tumor formation and how pathogens target cell polarity regulators for their own benefit.


 

 

Publications

 

A: Original Articles and Reviews 

Steinbacher T., D. Kummer and K. Ebnet (2017). Junctional adhesion molecule-A: functional diversity through molecular promiscuity. Cell Mol Life Sci. Dec 14. doi: 10.1007/s00018-017-2729-0. [Epub ahead of print].

Ebnet K. (2017). Junctional adhesion molecules (JAMs): Cell adhesion receptors with pleiotropic functions in cell physiology and development. Physiol. Rev. 97: 1529-1554.

Ebnet K., D. Kummer, T. Steinbacher, A. Singh, M. Nakayama and M. Matis (2017). Regulation of cell polarity by cell adhesion receptors. Sem. Cell Dev. Biol. Jul 22. pii: S1084-9521(17)30188-X. doi: 10.1016/j.semcdb.2017.07.032. [Epub ahead of print].

Fededa J.P., C. Esk, B. Mierzwa, R. Stanyte, S. Yuan, H. Zheng, K. Ebnet, W. Yan, J.A. Knoblich and D.W. Gerlich (2016). MicroRNA-34/449 controls mitotic spindle orientation during mammalian cortex development. EMBO J. 35: 2386–2398.

Brinkmann B.F., T. Steinbacher, C. Hartmann, D. Kummer, D. Pajonczyk, F. Mirzapourshafiyi, M. Nakayama, T. Weide, V. Gerke and K. Ebnet (2016). VE-cadherin interacts with cell polarity protein Pals1 to regulate vascular lumen formation. Mol. Biol. Cell27: 2811-2821.

Squarr A.J., K. Brinkmann, B. Chen, T. Steinbacher, K. Ebnet, M.K. Rosen and S. Bogdan (2016). Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. J. Cell Biol. 212: 591-603.

Franz J., B.F. Brinkmann, M. König, J. Hüve, C. Stock, K. Ebnet, C. Riethmüller (2016). Nanoscale Imaging Reveals a Tetraspanin-CD9 Coordinated Elevation of Endothelial ICAM-1 Clusters. PLoS One 11(1):e0146598. doi: 10.1371/journal.pone.0146598.

Tuncay H. and K. Ebnet (2016). Cell adhesion molecule control of planar spindle orientation. Cell Mol Life Sci. 73: 1195-207.

Tuncay H., B.F. Brinkmann and K. Ebnet (2015). Mitotic spindle orientation: JAM-A can fix it. Cell Cycle 14(24):3773-4. doi: 10.1080/15384101.2015.1105696.

Schwickert A., E. Weghake, K. Brüggemann, A. Engbers, B.F. Brinkmann, B. Kemper, J. Seggewiß, C. Stock, K. Ebnet, L. Kiesel, C. Riethmüller and M. Götte (2015). microRNA miR-142-3p inhibits breast cancer cell invasiveness by synchronous targeting of WASL, integrin alpha V, and Additional Cytoskeletal Elements. PLoS One 10(12):e0143993. doi: 10.1371/journal.pone.0143993.

Tuncay H., B.F. Brinkmann, T. Steinbacher, A. Schürmann, V. Gerke, S. Iden and K. Ebnet (2015). JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat Commun. 2015 Aug 26;6:8128. doi: 10.1038/ncomms9128.

Peddibhotla S.S.D., B.F. Brinkmann, D. Kummer, H. Tuncay, M. Nakayama, R. Adams, V. Gerke, and K. Ebnet (2013). Tetraspanin CD9 links junctional adhesion molecule-A to avb3 integrin to mediate bFGF-specific angiogenic signaling. Mol. Biol. Cell 24: 933-944.

Ebnet, K. (2012). JAM-A and aPKC: A close pair during cell-cell contact maturation and tight junction formation in epithelial cells. Tissue Barriers 1(1):e22993. doi: 10.4161/tisb.22993

Iden* S., S. Misselwitz*, S.S.D. Peddibhotla, H. Tuncay, D. Rehder, V. Gerke, H. Robenek, A. Suzuki and K. Ebnet (2012). aPKC phosphorylates JAM-A at Ser285 to promote cell contact maturation and tight junction formation. J. Cell Biol. 196: 623-639(* equal contribution).

Stelzer S., M.M. Worlitzer, L. Bahnassawy, K. Hemmer, K. Rugani, I. Werthschulte, A.L. Schön, B.F. Brinkmann, E.C. Bunk, T. Palm, K. Ebnet and J.C. Schwamborn (2012). JAM-C is an apical surface marker for neural stem cells. Stem Cells Dev. 21: 757-766.

Fabritz L, M.G. Hoogendijk, B.P. Scicluna, S.C. van Amersfoorth, L. Fortmueller, S. Wolf, S. Laakmann, N. Kreienkamp, I. Piccini, G. Breithardt, P.R. Noppinger, H. Witt, K. Ebnet, T. Wichter, B. Levkau, W.W. Franke, S. Pieperhoff, J.M. de Bakker, R. Coronel and P. Kirchhof (2011). Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice. J. Am. Coll. Cardiol.  57: 740-750.

Götte M., C. Mohr, C.-Y. Koo, C. Stock, A.-K. Vaske, M. Viola, S.A. Ibrahim, S. Peddibhotla, Y.H.-F. Teng, J.-Y. Low, K. Ebnet, L. Kiesel and G.W. Yip (2010). miR-145-dependent targeting of Junctional Adhesion Molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness. Oncogene  29: 6569-6580.

Stelzer S., K. Ebnet and J.C. Schwamborn (2010). JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain. BMC Neurosci. 11: 27, 1-9.

Ebnet K. (2008). Organization of multiprotein complexes at cell-cell junctions. Histochem. Cell Biol. 130:1-20.

Ebnet K., S. Iden, V. Gerke and A. Suzuki (2008). Regulation of epithelial and endothelial junctions by PAR proteins. Front. Biosci. 13: 6520-6536.

Pardo J., R. Wallich, K. Ebnet, S. Iden, H. Zentgraf, P. Martin, A. Ekiciler, A. Prins, A. Müllbacher, M. Huber and M.M. Simon (2007). Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin. Cell Death Differ. 14: 1768-1779.

Mandicourt G., S. Iden, K. Ebnet, M. Aurrand-Lions and B.A. Imhof (2007). JAM-C regulates tight junctions and integrin-mediated cell adhesion and migration. J. Biol Chem. 282: 1830-1837.

Iden S., D. Rehder, B. August, A. Suzuki, K. Wolburg-Buchholz, H. Wolburg, S. Ohno, J. Behrens, D. Vestweber and K. Ebnet (2006). A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells. EMBO Rep. 7: 1239-1246.

Rehder D., S. Iden, I. Nasdala, J. Wegener, M.K. Meyer Zu Brickwedde, D. Vestweber and K. Ebnet (2006). Junctional adhesion molecule-A participates in the formation of apico-basal polarity through different domains. Exp. Cell Res.312: 3389-3403.

Kondrashov A.V., M. Kiefmann, K. Ebnet, T. Khanam, R.S. Muddashetty and J. Brosius (2005). Inhibitory effect of naked neural BC1 RNA or BC200 RNA on eukaryotic in vitro translation systems is reversed by poly(A)-binding protein (PABP). J. Mol. Biol.353: 88-103.

Baumeister U., R. Funke, K. Ebnet, H. Vorschmitt, S. Koch and D. Vestweber (2005). Association of Csk to VE-cadherin and inhibition of cell proliferation. EMBO J.24: 1686-1695.

Wegmann F., K. Ebnet, L. Du Pasquier, D. Vestweber and S. Butz (2004). Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp. Cell Res.300: 121-133.

Gliki G., K. Ebnet, M. Aurrand-Lions, B.A. Imhof and R.H. Adams (2004) Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C. Nature431: 320-324.

Ebnet K., A. Suzuki, S. Ohno and D. Vestweber (2004). Junctional adhesion molecules (JAMs): More molecules with dual functions? J. Cell Sci.117: 19-29.

Ebnet K., M. Aurrand-Lions, A. Kuhn, F. Kiefer, S. Butz, K. Zander, M.K. Meyer Zu Brickwedde, A. Suzuki, B.A. Imhof and D. Vestweber (2003). The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: A possible role for JAMs in endothelial cell polarity. J. Cell Sci.116: 3879-3891.

Suzuki A., C. Ishiyama, K. Hashiba, M. Shimizu, K. Ebnet and S. Ohno (2002). aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization. J. Cell Sci.115: 3565-3573.

Nasdala I., K. Wolburg-Buchholz, H. Wolburg, A. Kuhn, K. Ebnet, G. Brachtendorf, U. Samulowitz, B. Kuster, B. Engelhardt, D. Vestweber and S. Butz (2002). A transmembrane tight junction protein selectively expressed on endothelial cells and platelets. J. Biol. Chem.277: 16294-16303.

Ebnet K., A. Suzuki, Y. Horikoshi, T. Hirose, M.K. Meyer Zu Brickwedde, S. Ohno and D. Vestweber (2001). The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM). EMBO J.20: 3738-3748.

Ebnet K., C.U. Schulz, M.K. Meyer Zu Brickwedde, G.G. Pendl and D. Vestweber (2000). Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1. J. Biol. Chem.275: 27979-27988.

Ebnet K. and D. Vestweber (1999). Molecular mechanisms that control leukocyte extravasation: the selectins and the chemokines. Histochem. Cell Biol.112: 1-23.

Simon M.M., M. Hausmann, T. Tran, K. Ebnet, J. Tschopp, R.T. Hla and A. Mullbacher (1997). In vitro- and ex vivo-derived cytolytic leukocytes from granzyme A x B double knockout mice are defective in granule-mediated apoptosis but not lysis of target cells. J. Exp. Med.186: 1781-1786.

Ebnet K., K.D. Brown, U.K. Siebenlist, M.M. Simon and S. Shaw S. (1997). Borrelia burgdorferi activates nuclear factor-kappa B and is a potent inducer of chemokine and adhesion molecule gene expression in endothelial cells and fibroblasts. J. Immunol.158: 3285-3292.

Ebnet K., M.M. Simon and S. Shaw (1996). Regulation of chemokine gene expression in human endothelial cells by proinflammatory cytokines and Borrelia burgdorferi. Ann. N. Y. Acad. Sci.797: 107-117.

Mullbacher A., K. Ebnet, R.V. Blanden, R.T. Hla, T. Stehle, C. Museteanu and M.M. Simon (1996). Granzyme A is critical for recovery of mice from infection with the natural cytopathic viral pathogen, ectromelia. Proc. Natl Acad. Sci. USA93: 5783-5787.

Ebnet K., E.P. Kaldjian, A.O. Anderson and S. Shaw (1996). Orchestrated information transfer underlying leukocyte endothelial interactions. Annu. Rev. Immunol.14: 155-177.

Ebnet K., M. Hausmann, F. Lehmann-Grube, A. Mullbacher, M. Kopf, M. Lamers and M.M. Simon (1995). Granzyme A-deficient mice retain potent cell-mediated cytotoxicity. EMBO J.14: 4230-4239.

Ebnet K., C.N. Levelt, T.T. Tran, K. Eichmann and M.M. Simon (1995). Transcription of granzyme A and B genes is differentially regulated during lymphoid ontogeny. J. Exp. Med.181: 755-763.

Dick T., G. Reichmann, K. Ebnet, M.M. Simon, H.P. Dienes, B. Echternacher, P.H. Krammer and A.B. Reske-Kunz (1993). An ovalbumin peptide-specific cytotoxic T cell clone with antigen self-presentation capacity uses two distinct mechanisms to kill target cells. Cell. Immunol.152: 333-347.

Ebnet K., M.D. Kramer and M.M. Simon (1992). Organization of the gene encoding the mouse T-cell-specific serine proteinase "granzyme A". Genomics13: 502-508.

Ebnet K., J. Chluba-de Tapia, U. Hurtenbach, M.D. Kramer and M.M. Simon (1991). In vivo primed mouse T cells selectively express T cell-specific serine proteinase-1 and the proteinase-like molecules granzyme B and C. Int. Immunol.3: 9-19.

Kramer M.D., S.E. Moter, M.M. Simon, K. Ebnet and R. Wallich (1990). Polymerase chain reaction in the demonstration of Borrelia burgdorferi DNA. Hautarzt41: 587-590.

Wallich R., S.E. Moter, M.M. Simon, K. Ebnet, A. Heiberger and M.D. Kramer (1990). The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect. Immun.58: 1711-1719.

 


B: Book Chapters

Brinkmann, B.F., H. Tuncay, and K. Ebnet (2015). Homotypic cell-cell interactions and apico-basal polarity in epithelial cells and endothelial cells. In “Cell Polarity 1: Biological role and basic mechanisms”. Ed. K. Ebnet, Springer International Publishing AG Switzerland, pp. 277-302

Ebnet K., B.F. Brinkmann, D. Kummer, S. Misselwitz, S.S.D. Peddibhotla, and H. Tuncay (2013). Tight junctions, Junctional Adhesion Molecules (JAMs), and the Blood Brain Barrier. In "Tight Junctions in Cancer Metastasis", Cancer Metastasis - Biology and Treatment 19. Ed. T.A. Martin and W.G. Jiang , Springer, Dordrecht-Heidelberg-New York-London, pp. 119-129.

Ebnet K., V. Gerke and S. Iden (2010). Cadherins and the regulation of tight junctions and cell polarity. In "Molecular and Functional Diversities of Cadherin and Protocadherin". Ed. K. Yoshida, Research Signpost, Kerala, India, pp. 57-68.

Ebnet K., V. Gerke and M. Aurrand-Lions (2010). Junctional Adhesion Molecules (JAMs). In "Modern Insights Into Disease From Molecules to Man. Adhesion Molecules". Ed. Victor R Preedy, Science Publishers, Enfield, USA, pp. 37-51.

Wolburg H., A. Lippoldt and K. Ebnet (2007). Tight junctions in the blood-brain barrier. In "Handbook of Neurochemistry and Molecular Neurobiology". Ed. A. Lajtha, Vol. 11: "Neural Membranes and Transport". Ed. M.E.A. Reith, Springer Berlin-Heidelberg, pp. 4-27.

Wolburg H., A. Lippoldt and K. Ebnet (2006). Tight junctions and the blood-brain barrier. In "Selected topics on tight junctions". Eds. L. Mariscal, RG Landes Bioscience, Georgetown, TX, USA, pp. 175-195.

Simon M.M., K. Ebnet, O. Gires, C. Museteanu, A. Siebers, T. Stehle, T.T. Tran, W. Zhong, L. Gern, M.D. Kramer and R. Wallich (1997). Lyme Borreliosis: progress in the development of a vaccine and new insights into the pathogenesis of the disease. In "Emerging and Re-Emerging Diseases Transmitted by Arthropod Vectors and Rodents; Proceedings of the 2nd Int. Symposium on Lyme Disease" in Japan". Eds. Y. Yanagihara and T. Masuzawa, pp. 245-259.

Sarin A., K. Ebnet, C.M. Zacharchuk and P.A. Henkart (1997). Caspase inhibitors as molecular probes of cell death. In "Programmed Cell Death: Proceedings of the 1996 International Symposium on Programmed Cell Death" in Shanghai. Eds. Y.B. Shi, Y. Shi, D.W. Scott, and Y. Xu, Plenum Press, New York, pp. 51-62.

Kramer M.D., U. Vettel, K. Ebnet and M.M. Simon (1993). The murine T cell-specific serine proteinase-1: cleavage activity on synthetic and natural substrates. In "Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods", chapter 59, p. 516-520. Eds. M. Sitkovsky and P. Henkart, Birkhäuser, Boston.

Simon M.M., K. Ebnet and M.D. Kramer (1993). Molecular analysis and possible pleiotropic function(s) of the T cell-specific serine proteinase-1 (TSP-1). In "Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods", chapter. 26, p. 278-294. Eds. M. Sitkovsky and P. Henkart, Birkhäuser, Boston.

 


C: Additional Publications

Vestweber D. and K. Ebnet (2001). Leukozyteneinwanderung in entzündete Gewebe – wie kommen die Abwehrzellen zum Wirkungsort? Biologen heute 25: 1-5.

Ebnet K. (1999). Immunüberwachung und Entzündung – ein komplexes Wechselspiel zwischen Leukozyten und Endothelzellen. Biologie in unserer Zeit 29: 26-35. 

 

 


PhysiolRev


EMBO


JCB

 

JCB

 

CM

 

NatComm

 

MBoC




1_Oncogene

2_HCB_2008

3_CDD_gen

4_JNC_2007

5_EMBORep_2006

6_JCS_2004

7_Nature_2004

8_JCS_2003

9_JCS_2002

10_EMBO_2001

11_JBC_2000

12_JEM_1997

13_JI_gen

14_PNAS_gen

15_JEM_1995

16_Gneomics_gen

 

 

Curriculum Vitae 

Since 2006
Independent Group Leader at the Institute of Medical Biochemistry, ZMBE, University of Münster, Germany

1996 - 2006
Institute of Cell Biology, ZMBE, University of Münster, Germany
Group Leader since 2002

1993 - 1996
Experimental Immunology Branch, NCI, NIH, Bethesda MD,  U.S.A.
 
1989 -1993
Max-Planck-Institute of Immunobiology, Freiburg i. Br., Germany

 

University Education and Degrees

2007
Professor (Apl.) at the Medical Faculty 
of the University Münster, Germany

2002

Habilitation: "Venia legendi" in Immunology, Medical Faculty of the University Münster, Germany

1992
PhD (Dr. rer. nat.), University Freiburg, Germany

1989
Masters in Biology, University Stuttgart-Hohenheim, Germany


Previous and Current Research


▪ Cell-cell contact formation
▪ Cell polarity in epithelial and endothelial cells
▪ Junctional Adhesion Molecules (JAMs) and Cadherins in cell polarity formation
▪ Chemokines and adhesion molecules in endothelial cells
▪ Inflammatory responses in endothelial cells
▪ Apoptosis-inducing serine proteases (Granzymes) of cytotoxic T lymphocytes