CV & Publications

 

Prof. Dr. Klaus Ebnet
Group Leader Institute-Associated Research Group "Cell Adhesion and Cell Polarity"
Institute of Medical Biochemistry
ZMBE

News

 

We have edited a book on Cell Polarity.

The book comes in 2 volumes:

 

Volume 1(Biological Role and Basic Mechanisms)describes the molecular toolkit that is used to generate cell polarityand how this limited set of polarity molecules regulates variousaspects of cell polarity in different cell types.

 

Volume 2(Role in Development and Disease)puts a major emphasis on the physiological role of cell polarity anddescribes the role of cell polarity during development and tumorformation and how pathogens target cell polarity regulators for theirown benefit.


 

 

Publications

A: Original Articles and Reviews

Thölmann S, Seebach J, Otani T, Florin L, Schnittler H, Gerke V, Furuse M and Ebnet K (2022). JAM-A interacts with α3β1 integrin and tetraspanins CD151 and CD9 to regulate collective cell migration of polarized epithelial cells. Cell. Mol. Life Sci. Accepted for publication.

Haas AJ, Zihni C, Ruppel A, Hartmann C, Ebnet K, Tada M, Balda MS and Matter K (2020). Interplay between extracellular matrix stiffness and JAM-A regulates mechanical load on ZO-1 and tight junction assembly. Cell Rep. 32(3):107924. doi: 10.1016/j.celrep.2020.107924.

Hartmann C, Schwietzer YA, Kummer D, Kirschnick N, Hoppe E, Thüring EM, Glaesner-Ebnet M, Brinkmann F, Gerke V, Reuter S, Nakayama M and Ebnet K (2020). The mitochondrial outer membrane protein SYNJ2BP interacts with the cell adhesion molecule TMIGD1 and can recruit it to mitochondria. BMC Mol Cell Biol. 2020 Apr 17;21(1):30. doi: 10.1186/s12860-020-00274-1.

Kummer D,  Steinbacher T, Schwietzer MF, Thölmann S and Ebnet K (2020). Tetraspanins: Integrating cell surface receptors to functional microdomains in homeostasis and disease. Med.Microbiol.Immunol. 209(4)397-405. doi: 10.1007/s00430-020-00673-3.

Hartmann C, Schwietzer YA, Otani T, Furuse M and Ebnet K (2020). Physiological functions of Junctional Adhesion Molecules (JAMs) in tight junctions. BBA Biomembranes.1862(9):183299. doi: 10.1016/j.bbamem.2020.183299.

Otani T, Nguyen TP, Tokuda S, Sugihara K, Sugawara T, Furuse K, Miura T, Ebnet K and Furuse M (2019). Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity. J. Cell Biol. 218(10):3372-3396.

Merlen G, Kahale N, Ursic-Bedoya J, Bidault-Jourdainne V, Simerabet H, Doignon I, Tanfin Z, Garcin I, Péan N, Gautherot J, Davit-Spraul A, Guettier C, Humbert L, Rainteau D, Ebnet K, Ullmer C, Cassio D and Tordjmann T (2020). TGR5-dependent hepatoprotection through the regulation of biliary epithelium barrier function. Gut 69: 146-157.

Riddell M, Nakayama A, Hikita T, Mirzapourshafiyi F, Kawamura T, Pasha A, Li M, Masuzawa M, Looso M, Steinbacher T, Ebnet K, Potente M, Hirose T, Ohno S, Fleming I, Gattenlöhner S, Aung PP, Phung T, Yamasaki O, Yanagi T, Umemura H and Nakayama M.(2018). aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA binding ability. Nat Commun. 2018 Dec 17;9(1):5357. doi: 10.1038/s41467-018-07739-0.

Steinbacher T and Ebnet K (2018). The regulation of junctional actin dynamics by cell adhesion receptors. Histochem. Cell Biol. 150(4):341-350.

Kummer D  and Ebnet K (2018). Junctional Adhesion Molecules (JAMs): The JAM - Integrin connection. Cells. Mar 26;7(4). pii: E25. doi: 10.3390/cells7040025.

Steinbacher T, Kummer D  and Ebnet K (2018). Junctional Adhesion Molecule-A: Functional diversity through molecular promiscuity. Cell. Mol. Life Sci.75:1393–1409. doi: 10.1007/s00018-017-2729-0.

Ebnet K (2017). Junctional adhesion molecules (JAMs): Cell adhesion receptors with pleiotropic functions in cell physiology and development. Physiol. Rev. 97: 1529-1554. doi: 10.1152/physrev.00004.2017.

Ebnet K, Kummer D, Steinbacher T, Singh A, Nakayama M and Matis M (2018). Regulation of cell polarity by cell adhesion receptors. Sem. Cell Dev. Biol. 81: 2-12. doi:10.1016/j.semcdb.2017 .07.032.

Fededa JP, Esk C, Mierzwa B, Stanyte R, Yuan S, Zheng H, Ebnet K, Yan W, Knoblich JA and Gerlich DW (2016). MicroRNA-34/449 controls mitotic spindle orientation during mammalian cortex development. EMBO J. 35: 2386–2398.

Brinkmann BF, Steinbacher T, Hartmann C, Kummer D, Pajonczyk D, Mirzapourshafiyi F, Nakayama M, Weide T, Gerke V and Ebnet K (2016). VE-cadherin interacts with cell polarity protein Pals1 to regulate vascular lumen formation. Mol.Biol.Cell 27: 2811-2821.

Squarr AJ, Brinkmann K, Chen B, Steinbacher T, Ebnet K, Rosen MK and Bogdan S (2016). Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. J. Cell Biol. 212: 591-603.

Franz J, Brinkmann BF, König M, Hüve J, Stock C, Ebnet K and Riethmüller C (2016). Nanoscale imaging reveals a tetraspanin-CD9 coordinated elevation of endothelial ICAM-1 clusters. PLoS ONE 11(1):e0146598. doi: 10.1371/journal.pone.0146598.

Tuncay H and Ebnet K (2016). Cell adhesion molecule control of planar spindle orientation. Cell. Mol. Life Sci. 73(6):1195-207. doi: 10.1007/s00018-015-2116-7.

Tuncay H, Brinkmann BF and Ebnet K (2015). Mitotic spindle orientation: JAM-A can fix it. Cell Cycle 14(24): 3773-3774.

Schwickert A, Weghake E, Brüggemann K, Engbers A, Seggewiß J, Kemper B, Stock C, Brinkmann BF, Ebnet K, Kiesel L, Riethmüller C and Götte M (2015). microRNA miR-142-3p inhibits breast cancer cell invasiveness by synchronous targeting of WASL, integrin alpha V, and additional cytoskeletal elements. PLoS ONE 10(12): e0143993. doi:10.1371/journal.pone.0143993.

Tuncay H., Brinkmann BF, Steinbacher T, Schürmann A, Gerke V, Iden S and Ebnet K (2015). JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat Commun. 2015 Aug 26;6:8128. doi: 10.1038/ncomms9128.

Peddibhotla S.S.D., Brinkmann BF, Kummer D, Tuncay H, Nakayama M, Adams R, Gerke V and Ebnet K (2013). Tetraspanin CD9 links junctional adhesion molecule-A to avb3 integrin to mediate bFGF-specific angiogenic signaling. Mol. Biol. Cell 24: 933-944.

Ebnet K (2012). JAM-A and aPKC: A close pair during cell-cell contact maturation and tight junction formation in epithelial cells. Tissue Barriers 1(1):e22993. doi: 10.4161/tisb.22993.

Iden* S, Misselwitz S*, Peddibhotla SSD, Tuncay H, Rehder D, Gerke V, Robenek H, Suzuki A and Ebnet K (2012). aPKC phosphorylates JAM-A at Ser285 to promote cell contact maturation and tight junction formation. J. Cell Biol. 196: 623-639. (*equal contribution).

Stelzer S, Worlitzer MM, Bahnassawy L, Hemmer K, Rugani K, Werthschulte I, Schön AL, Brinkmann BF, Bunk EC, Palm T, Ebnet K and Schwamborn JC (2012). JAM-C is an apical surface marker for neural stem cells. Stem Cells Dev. 21: 757-766.

Fabritz L, Hoogendijk MG, Scicluna BP, van Amersfoorth SC, Fortmueller L, Wolf S, Laakmann S, Kreienkamp N, Piccini I, Breithardt G, Noppinger PR, Witt H, Ebnet K, Wichter T, Levkau B, Franke WW, Pieperhoff S, de Bakker JM, Coronel R and Kirchhof P (2011). Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice. J. Am. Coll. Cardiol.  57: 740-750.

Götte M, Mohr C, Koo C-Y, Stock C, Vaske A-K, Viola M, Ibrahim SA, Peddibhotla S, Teng YH-F, Low J-Y, Ebnet K, Kiesel L and Yip GW (2010). miR-145-dependent targeting of Junctional Adhesion Molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness. Oncogene  29: 6569-6580.

Stelzer S, Ebnet K and Schwamborn JC (2010). JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain. BMC Neurosci. 11: 27, 1-9.

Ebnet K (2008). Organization of multiprotein complexes at cell-cell junctions. Histochem. Cell Biol. 130: 1-20.

Ebnet K, Iden S, Gerke V and Suzuki A (2008). Regulation of epithelial and endothelial junctions by PAR proteins. Front. Biosci. 13: 6520-6536.
Pardo J, Wallich R, Ebnet K, Iden S, Zentgraf H, Martin P, Ekiciler A, Prins A, Müllbacher A, Huber M and Simon MM (2007). Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin. Cell Death Differ. 14: 1768-1779.

Mandicourt G, Iden S, Ebnet K, Aurrand-Lions M and Imhof BA (2007). JAM-C regulates tight junctions and integrin-mediated cell adhesion and migration. J. Biol Chem. 282: 1830-1837.

Iden S, Rehder D, August B, Suzuki A, Wolburg-Buchholz K, Wolburg H, Ohno S, Behrens J, Vestweber D and Ebnet K (2006). A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells. EMBO Rep. 7: 1239-1246.

Rehder D, Iden S, Nasdala I, Wegener J, Meyer Zu Brickwedde MK, Vestweber D and Ebnet K (2006). Junctional adhesion molecule-A participates in the formation of apico-basal polarity through different domains. Exp. Cell Res. 312: 3389-3403.

Kondrashov AV, Kiefmann M, Ebnet K, Khanam T, Muddashetty RS and Brosius J (2005). Inhibitory effect of naked neural BC1 RNA or BC200 RNA on eukaryotic in vitro translation systems is reversed by poly(A)-binding protein (PABP). J. Mol. Biol. 353: 88-103.

Baumeister U, Funke R, Ebnet K, Vorschmitt H, Koch S and Vestweber D (2005). Association of Csk to VE-cadherin and inhibition of cell proliferation. EMBO J. 24: 1686-1695.

Wegmann F, Ebnet K, Du Pasquier L, Vestweber D and Butz S (2004). Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp. Cell Res. 300: 121-133.

Gliki G, Ebnet K, Aurrand-Lions M, Imhof BA and Adams RH (2004) Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C. Nature 431: 320-324.

Ebnet K, Suzuki A, Ohno S and Vestweber D (2004). Junctional adhesion molecules (JAMs): More molecules with dual functions? J. Cell Sci. 117: 19-29.

Ebnet K, Aurrand-Lions M, Kuhn A, Kiefer F, Butz S, Zander K, Meyer Zu Brickwedde MK, Suzuki A, Imhof BA and Vestweber D (2003). The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: A possible role for JAMs in endothelial cell polarity. J. Cell Sci. 116: 3879-3891.

Suzuki A, Ishiyama C, Hashiba K, Shimizu M, Ebnet K and Ohno S (2002). aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization. J. Cell Sci. 115: 3565-3573.

Nasdala I, Wolburg-Buchholz K, Wolburg H, Kuhn A, Ebnet K, Brachtendorf B, Samulowitz U, Kuster B, Engelhardt B, Vestweber D and Butz S (2002). A transmembrane tight junction protein selectively expressed on endothelial cells and platelets. J. Biol. Chem. 277: 16294-16303.

Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu Brickwedde MK, Ohno S and Vestweber D (2001). The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM). EMBO J. 20: 3738-3748.

Ebnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG and Vestweber D (2000). Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1. J. Biol. Chem. 275: 27979-27988.

Ebnet K and Vestweber D (1999). Molecular mechanisms that control leukocyte extravasation: the selectins and the chemokines. Histochem. Cell Biol. 112: 1-23.

Simon MM, Hausmann M, Tran T, Ebnet K, Tschopp J, Hla RT and Mullbacher A (1997). In vitro- and ex vivo-derived cytolytic leukocytes from granzyme A x B double knockout mice are defective in granule-mediated apoptosis but not lysis of target cells. J. Exp. Med. 186: 1781-1786.

Ebnet K, Brown KD, Siebenlist UK, Simon MM and Shaw S (1997). Borrelia burgdorferi activates nuclear factor-kappa B and is a potent inducer of chemokine and adhesion molecule gene expression in endothelial cells and fibroblasts. J. Immunol. 158: 3285-3292.

Ebnet K, Simon MM and Shaw S (1996). Regulation of chemokine gene expression in human endothelial cells by proinflammatory cytokines and Borrelia burgdorferi. Ann. N. Y. Acad. Sci. 797: 107-117.

Mullbacher A, Ebnet K, Blanden RV, Hla RT, Stehle T, Museteanu C and Simon MM (1996). Granzyme A is critical for recovery of mice from infection with the natural cytopathic viral pathogen, ectromelia. Proc. Natl Acad. Sci. USA 93: 5783-5787.

Ebnet K, Kaldjian EP, Anderson AO and Shaw S (1996). Orchestrated information transfer underlying leukocyte endothelial interactions. Annu. Rev. Immunol. 14: 155-177.

Ebnet K, Hausmann M, Lehmann-Grube F, Mullbacher A, Kopf M, Lamers M and Simon MM (1995). Granzyme A-deficient mice retain potent cell-mediated cytotoxicity. EMBO J. 14: 4230-4239.

Ebnet K, Levelt CN, Tran TT, Eichmann K and Simon MM (1995). Transcription of granzyme A and B genes is differentially regulated during lymphoid ontogeny. J. Exp. Med. 181: 755-763.

Dick T, Reichmann G, Ebnet K, Simon MM, Dienes HP, Echternacher B, Krammer PH and Reske-Kunz AB (1993). An ovalbumin peptide-specific cytotoxic T cell clone with antigen self-presentation capacity uses two distinct mechanisms to kill target cells. Cell. Immunol. 152: 333-347.

Ebnet K, Kramer MD and Simon MM (1992). Organization of the gene encoding the mouse T-cell-specific serine proteinase "granzyme A". Genomics 13: 502-508.

Ebnet K, Chluba-de Tapia J, Hurtenbach U, Kramer MD and Simon MM (1991). In vivo primed mouse T cells selectively express T cell-specific serine proteinase-1 and the proteinase-like molecules granzyme B and C. Int. Immunol. 3: 9-19.

Kramer MD, Moter SE, Simon MM, Ebnet K and Wallich R (1990). Polymerase chain reaction in the demonstration of Borrelia burgdorferi DNA. Hautarzt 41: 587-590.

Wallich R, Moter SE, Simon MM, Ebnet K, Heiberger A and Kramer MD (1990). The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect. Immun. 58: 1711-1719.
 

B: Book Chapters

Brinkmann BF, Tuncay H and Ebnet K (2015). Homotypic cell-cell interactions and apico-basal polarity in epithelial cells and endothelial cells. In “Cell Polarity 1: Biological role and basic mechanisms”. Ed. K. Ebnet, Springer International Publishing AG Switzerland, pp. 277-302

Ebnet K, Brinkmann BF, Kummer D, Misselwitz S, Peddibhotla SSD and Tuncay H (2013). Tight junctions, Junctional Adhesion Molecules (JAMs), and the Blood Brain Barrier. In "Tight Junctions in Cancer Metastasis", Cancer Metastasis - Biology and Treatment 19. Ed. T.A. Martin and W.G. Jiang , Springer, Dordrecht-Heidelberg-New York-London, pp. 119-129.

Ebnet K, Gerke V and Iden S (2010). Cadherins and the regulation of tight junctions and cell polarity. In "Molecular and Functional Diversities of Cadherin and Protocadherin". Ed. K. Yoshida, Research Signpost, Kerala, India, pp. 57-68.

Ebnet K, V. Gerke and M. Aurrand-Lions (2010). Junctional Adhesion Molecules (JAMs). In "Modern Insights Into Disease From Molecules to Man. Adhesion Molecules". Ed. Victor R Preedy, Science Publishers, Enfield, USA, pp. 37-51.

Wolburg H, Lippoldt A and Ebnet K (2007). Tight junctions in the blood-brain barrier. In "Handbook of Neurochemistry and Molecular Neurobiology". Ed. A. Lajtha, Vol. 11: "Neural Membranes and Transport". Ed. M.E.A. Reith, Springer Berlin-Heidelberg, pp. 4-27.

Wolburg H, Lippoldt A and Ebnet K (2006). Tight junctions and the blood-brain barrier. In "Selected topics on tight junctions". Eds. L. Mariscal, RG Landes Bioscience, Georgetown, TX, USA, pp. 175-195.

Simon MM, Ebnet K, Gires O, Museteanu C, Siebers A, Stehle T, Tran TT, Zhong W, Gern L, Kramer MD and Wallich R (1997). Lyme Borreliosis: progress in the development of a vaccine and new insights into the pathogenesis of the disease. In "Emerging and Re-Emerging Diseases Transmitted by Arthropod Vectors and Rodents; Proceedings of the 2nd Int. Symposium on Lyme Disease" in Japan". Eds. Y. Yanagihara and T. Masuzawa, pp. 245-259.

Sarin A, Ebnet K, Zacharchuk CM and Henkart PA (1997). Caspase inhibitors as molecular probes of cell death. In "Programmed Cell Death: Proceedings of the 1996 International Symposium on Programmed Cell Death" in Shanghai. Eds. Y.B. Shi, Y. Shi, D.W. Scott, and Y. Xu, Plenum Press, New York, pp. 51-62.

Kramer MD, Vettel U, Ebnet K and Simon MM (1993). The murine T cell-specific serine proteinase-1: cleavage activity on synthetic and natural substrates. In "Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods", chapter 59, p. 516-520. Eds. M. Sitkovsky and P. Henkart, Birkhäuser, Boston.#

Simon MM, Ebnet K and Kramer MD (1993). Molecular analysis and possible pleiotropic function(s) of the T cell-specific serine proteinase-1 (TSP-1). In "Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods", chapter. 26, p. 278-294. Eds. M. Sitkovsky and P. Henkart, Birkhäuser, Boston.
 

C: Additional Publications

Vestweber D and Ebnet K (2001). Leukozyteneinwanderung in entzündete Gewebe – wie kommen die Abwehrzellen zum Wirkungsort? Biologen heute 25: 1-5.

Ebnet K (1999). Immunüberwachung und Entzündung – ein komplexes Wechselspiel zwischen Leukozyten und Endothelzellen. Biologie in unserer Zeit 29: 26-35.

 

 


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PhysiolRev


EMBO


JCB

 

JCB

 

CM

 

NatComm

 

MBoC




1_Oncogene

2_HCB_2008

3_CDD_gen

4_JNC_2007

5_EMBORep_2006

6_JCS_2004

7_Nature_2004

8_JCS_2003

9_JCS_2002

10_EMBO_2001

11_JBC_2000

12_JEM_1997

13_JI_gen

14_PNAS_gen

15_JEM_1995

16_Gneomics_gen

 

 

Curriculum Vitae 

Since 2006
Independent Group Leader at the Institute of Medical Biochemistry, ZMBE, University of Münster, Germany

1996 - 2006
Institute of Cell Biology, ZMBE, University of Münster, Germany
Group Leader since 2002

1993 - 1996
Experimental Immunology Branch, NCI, NIH, Bethesda MD,  U.S.A.
 
1989 -1993
Max-Planck-Institute of Immunobiology, Freiburg i. Br., Germany

 

University Education and Degrees

2007
Professor (Apl.) at the Medical Faculty 
of the University Münster, Germany

2002

Habilitation: "Venia legendi" in Immunology, Medical Faculty of the University Münster, Germany

1992
PhD (Dr. rer. nat.), University Freiburg, Germany

1989
Masters in Biology, University Stuttgart-Hohenheim, Germany


Previous and Current Research


▪ Cell-cell contact formation
▪ Cell polarity in epithelial and endothelial cells
▪ Junctional Adhesion Molecules (JAMs) and Cadherins in cell polarity formation
▪ Chemokines and adhesion molecules in endothelial cells
▪ Inflammatory responses in endothelial cells
▪ Apoptosis-inducing serine proteases (Granzymes) of cytotoxic T lymphocytes