| HUSEC002 |
| Strain no. | Serotype | ST(CC) | Phylogenetic group |
| 5152/97 | Ont:H- | 330 (10) | A |
| Molecular characteristics (virulence and fitness genes) |
| | | | | | | | | lpfO157 | lpfO157 | | |
| stx | eae | EHEC-hlyA | efa1 | iha | sfpA | lpfO26 | lpfO113 | OI-141 | OI-154 | saa | terE |
| 2 | +* | + | - | - | - | - | - | - | - | - | + |
|
| | | | Stx titer | "Antibiotic resistance pattern (R, resistant I, intermediate S, susceptible)" |
| GUD | EHEC-Hly | CT-SMAC | mean 1: | AMP |
| + | + | + | 256 | I |
| |
| AMC | MEZ | PRL | CXM | CTX | CAZ | CFS | E | TE | CN | TOB | AK | OFX | CIP | SXT | ATM | CT
| |
| R | S | S | I | S | S | R | R | S | I | I | S | S | S | S | S | S | |
| Comment |
*The eae gene in strain 5152/97, which is located between map and espF, was detectable by DNA–DNA hybridization but not by PCR. Interestingly, a long-distance map-espF PCR resulted in a fragment of ~4 kb instead of the predicted 12.7 kb in reference strain EDL933, indicating a truncation that affects eae (Creuzburg et al. (2011) Evolutionary analysis and distribution of type III effector genes in pathogenic Escherichia coli from human, animal and food sources. Environ Microbiol 13: 439-452).
|
| Legend |
| | | |
| Gene | Product (marker for) | |
| stx | Shiga toxin | |
| eae | intimin | |
| EHEC-hlyA | EHEC hemolysin | |
| efa1 | EHEC factor for adherence (Efa1) | |
| iha | iron-regulated gene A homologue adhesin (Iha) | |
| sfpA | major subunit of Sfp fimbriae | |
| lpfAO26 | major subunit of long polar fimbriae of EHEC O26 | |
| lpfAO113 | major subunit of long polar fimbriae of EHEC O113 | |
| lpfAO157-OI141 | major subunit of long polar fimbriae of EHEC O157 located on O island 141 | |
| lpfAO157-OI154 | major subunit of long polar fimbriae of EHEC O157 located on O island 154 | |
| saa | STEC autoagglutinating adhesin | |
| terE | marker for the ter cluster encoding tellurite resistance | |