Membranes define the boundaries of cells and their internal organelles and, consequently, are fundamental to compartmentalization of the chemical reactions that sustain the living state. Importantly, cellular membranes do not behave as homogenous two-dimensional fluids. On the contrary, they are heterogeneous entities that contain various structures on the nano-mesoscale, where particular membrane molecules stay together for a certain amount of time to influence fundamental cellular processes such as nutrient uptake, signaling, and endo/exocytosis. Another defining feature of cellular membranes is an astonishing diversity of building blocks. This workshop focuses on the molecular principles that govern the formation of functional subdomains in cellular membranes. The different sessions deal with the composition, dynamics and turnover of membrane subdomains, how these structural entities execute their specialized tasks, and how membrane patterning in general relies on a complex, often poorly understood interplay between proteins and lipids. A substantial part of the program is dedicated to defining the principal challenges and technological innovations relevant to the study of membrane subdomains. The latter include ongoing developments in super-resolution microscopy and in the analysis of protein-lipid interaction networks.