Protein gel electrophoresis

Picture courtesy of GE

We perform gel electrophoretic experiments in 1 und 2 dimensions according to established protocols or your settings (1/2-PAGE). To that end, both Bio-Rad, Amersham and Gelcompany/Serva devices are available.

We have also several years of experience with the DIGE-system for differential protein expression analysis.

Necessary protein amounts:

  • 0.5-1 mg for Coomassie staining
  • 0.15 mg for analytical DIGE gels (0.075 mg each for control and sample)
  • 0.5 mg for preparative DIGE gels

Limits of detection:

  • Coomassie R250 30-100 ng
  • colloidal Coomassie G250 1-16 ng
  • silver 1-10 ng
  • fluorescence 0.1-5 ng
Fluorescence detection
Typhoon 9400 is a high-performance fluorescence scanner with 3 lasers (457/488, 532, 633 nm) for various applications such as the detection of phosphoproteins using Diamond Pro Q stain.

DIGE analysis of the IFG in collaborations:
Lewin, G., Matus, M., Basu, A., Frebel, K., Rohsbach, S.P., Safronenko, A., Seidl, M.D., Stümpel, F., Buchwalow, I., König, S., Engelhardt, S., Lohse, M. J., Schmitz, W., Müller, F.U., Critical role of transcription factor cyclic AMP response element modulator in beta1-adrenoceptor-mediated cardiac dysfunction. Circulation 2009, 119:79-88

Arias-Loza, P.-A., Hu, K., Dienesch, C., König, S., Mehlich, A.M., Jazbutyte, V., Fritzemeier, K.-H.,  Hegele-Hartung, C., Neyses, L., Theo Pelzer, T. Both estrogen receptor subtypes, ER alpha and ER beta, attenuate cardiovascular remodeling in aldosteron-salt treated rats. Hypertension (2007) 50:432-438