Fluorescence detection

High-performance scanner Typhoon 9400 3-laser scanner for automatic 4-colour fluorescence detection, direct chemoluminescence (50 ng), storage phosphor, quantum dots (TLC-plates)Excitation: 532 nm Nd:YAG, 633 nm HeNe, 457/488 nm Ar
Filter: 7 emission filter, 3 beam splitter, up to 13 emission filter positions
Samples: gels, gel sandwiches, blots, microtiterplates, TLC-plates, macroarrays Examples and settings:
  • Detection of nucleic acids in the gel, SYBR Gold, SYBR Green I/II, Vista Green (488 nm, 520 BP 40)
  • Quantification of nucleic acids in solution, OliGreen, PicoGreen, RiboGreen (488/532 nm, 520 BP 40 / 526 SP)
  • Southern and Northern blotting, ODDAO Phosphate (633 nm, 670 BP 30), ECF (532 nm, 526 SP)
  • Differential display
  • Bandshift assay (gel mobility shift assay, gel retardation assay)
  • Detection of proteins in the gel, SYPRO Orange (488/532 nm, 555 BP 20), SYPRO Red (532 nm, 610 BP 30), SYPRO Ruby / IEF (457/488/532 nm, 610 BP 30), SYPRO Tangerine (488/532 nm, 610 BP 30), Pro-Q Diamond phosphoprotein stain / Pro-Q Emerald glycoprotein stain
  • Quantification of proteins in solution, CBQCA (488/532 nm, 555 BP 20), Nano Orange (457/488/532 nm, 580 BP 30)
  • Western blotting, DDDAO Phosphate (633 nm, 670 BP 30), ECF (532 nm, 526 SP), ECF Plus (457 nm, 520 BP 40), Fluorescin (532 nm, 526 SP), Cy3 (532 nm, 580 BP 30), Cy5 (633 nm, 670 BP 30), Alexa Fluor 532 (532 nm, 555 BP 20), Alexa Fluor 633 und PBXL-3 (633 nm, 670 BP 30), SYPRO Ruby blot (457/488/532 nm, 610 BP 30)
  • Natural fluorescent proteins, Allophycocyanin (633 nm, 670 BP 30), B- und R-phycoerythrin (532 nm, 580 BP 30), GFP (wt) und –S65T / EGFP (488 nm, 520 BP 40), EYFP (488/532 nm, 520 BP 20 / 526 SP), DsRed (532 nm, 580 BP 30)
  • Further labels, Alexa Fluor 488 (488 nm, 520 BP 40), Alexa Fluor 546 (532 nm, 580 BP 30), Alexa Fluor 568 / 594 (532 nm, 610 BP 30), Alexa Fluor 660 / 680 (633 nm, 670 BP 30), BODIPY 630/650 / 650/665 (633 nm, 670 BP 30), BODIPY FL (488 nm, 520 BP 40), BODIPY TMR-X (532 nm, 580 BP 30), BODIPY TR-X (532 nm, 610 BP 30), Cy2 (488 nm, 520 BP 40), Cy3.5 (532 nm, 610 BP 30), Cy5.5 (633 nm, 670 BP 30), FAM / FITC / FluorX / Oregon Green 488 (488 nm, 520 BP 40), HEX / JOE / TET / Oregon Green 514 (532 nm, 555 BP 20), Rhodamine Green (488/532 nm, 520 BP 40 / 526 SP), Rhodamine Red-X / TAMRA / Tetramethylrhodamine (580 nm, BP 30), ROX / Texas Red-X (532 nm, 610 BP 30)