Assignments
Broad methodical spectrum on the fields of- molecular biology (classical cloning, PCR, mutagenesis etc.)- protein biochemistry (protein purification and quantification, protein separation (IEF and SDS-PAGE electrophoresis), Western Blotting, 2D gel electrophoresis, different chromatography techniques, cell fractionations, immunoprecipitation, etc.)- cell biology (mammalian cell culture, maintenance of cells, different transfection methods, generation of stable cell lines, lentiviral transduction, FACS analysis etc.)- yeast microbiology (transformation, endogenous tagging or knock-out of yeast genes, protein relocation)- basic skills in imaging techniques (epifluorescence, confocal, TIRF)Further functions in the group: Lab management (ordering and inventory of chemicals, consumables etc., authorized person for dangerous goods, collection of methodical protocols)