This year's conference will focus on imaging using MALDI mass spectrometry. An entire afternoon (Nov 29) will be dedicated to showcase such experiments in a workshop organized by Klaus Dreisewerd and his colleagues together with Waters Corporation at the Institute of Hygiene. Prof. Dr. Ron Heeren, Maastricht MultiModal Molecular Imaging Institute (M4I), well known for his ground-breaking research in this field will elaborate on this topic in his keynote lecture on Nov 30. Other leading manufacturers in the field of MALDI imaging instrumentation and protein analysis - Bruker, PALL FortéBio, SCiLS and SERVA Electrophoresis will further illustrate the current state-of-the-art in talks and hands-on-workshops.This is an excellent opportunity for students and PIs alike to improve their understanding and discuss and troubleshoot with the experts. We encourage you to use this great opportunity to train your students and meet the research leaders.
Fortebio
Biolayer Interferometry (BLI) (November 29)Venue: Seminar room Core Unit Proteomics (ZMBE 1st floor, Von-Esmarch-St. 56, side entrance ZMBE from the courtyard)Abstract: Biolayer Interferometry allows rapid, real-time and label-free molecular interaction analysis. Many applications can be run on a single platform, from small molecule assays to large molecule-, liposome- and virus interaction analyses. Further, functional cellular responses can be detected by cBLI assays. Full kinetic characterization and protein quantification is possible, even from crude samples. The throughput is easily scalable from single to 96 parallel samples, the Dip-and Read approach allows sample recovery for additional experiments, and the microfluidics- free instrumentation requires little-to-no maintenance. BLI is the method of choice to replace time- consuming techniques like ELISA, Western Blotting or analytical HPLC.
10:30 | Workshop Registration |
11:00 | Stefan Scory, Fortebio Biolayer Interferometry (BLI) – a label-free, biosensor-based technology to monitor molecular interactions and signal transduction in living cells Information is given on quantitative and kinetic analysis in sensitive affinity experiments |
12:00 | Lunch (not provided) |
WORKSHOP Institute of Hygiene and Waters GmbH
MALDI imaging(November 29)Venue: Lecture hall (HS) at Robert‐Koch‐Str. 31 and laboratories at the Institute of Hygiene (Biomedical Mass Spectrometry) at Robert‐Koch‐Str. 41/51
13:00 | Workshop Registration |
13:15 | Klaus Dreisewerd, Simone König Welcome address |
13:20 | Michael Batey, Waters Corporation, Wilmslow, UK Molecular Imaging by Mass Spectrometry: Applications in Biomedical Research and Drug Discovery |
14:20 | Klaus Dreisewerd, University of Münster Brief introduction to MALDI‐MS imaging and MALDI‐2‐MS imaging |
15:00 | Coffee break and lab tour in small groups |
16:15 | Demonstration of experiments and data evaluation strategies via Team Viewer
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18:00 | Closing remarks |
TALK Bruker
MALDI Imaging(November 30)Abstract: The presentation will highlight Bruker´s latest developments in MALDI imaging mass spectrometry. Complementary MALDI imaging solutions based on either MALDI-TOF/TOF (Bruker rapiflex) or MALDI-FTICR (Bruker solarix 2xR) will be highlighted. The rapiflex MALDI-TOF/TOF technology allows to perform MALDI imaging at an unparalleled level of speed and spatial definition, whereas solarix 2xR facilitates MSI at incredibly high mass resolution and accuracy. The individual merits of both of these systems will be illustrated by application examples covering various research areas.
13:30 - 14:00 | Arndt Asperger Complementary MALDI imaging solutions based on latest MALDI-TOF and –FTICR MS technology |
WORKSHOP Waters GmbH
Protein analysis(November 30)
14:00 - 15:00 | Marc Kipping, Waters GmbH An Overview of Past, Present and Future of Data Independent Acquisition Strategies (covers LC-MSe, Ion Mobiltity Spectrometry (IMS), HDMSe, Stepped Quadrupole, SONAR) Introduction to modern mass spectrometry techniques also performed with your samples at the Core Unit Proteomics. A great opportunity to ask your questions about our experiments. |
15:00 - 15:30 | Gottfried Pohlentz, University of Münster Efficient analysis of proteolytic peptides and glycopeptides by use of ion mobility separations (IMS) and subsequent low energy collision-induced dissociation |
WORKSHOP Serva Electrophoresis
Protein electrophoresis for professionals(November 30)
15:30 - 16:30 | Günter Thesseling, Reiner Westermeier, Serva Electrophoresis Protein electrophoresis (basics, overview of different techniques, visualisation of protein separation, trouble shooting) The opportunity to learn about and discuss the beloved and hated workhorse in the biochemical laboratory, gel electrophoresis, with the experts and text book authors. |