Gene Expression Profiling

Beside NGS-based transcriptome analysis, the Core Facility provides a multitude of approaches to determine gene expression pattern.

Two real-time PCR systems (7900HT, 7500) enable qPCR methods to detect your transcripts.  In addition, we are able to process 384-well TaqMan® arrays (micro fluidic card).

The direct hybridization assay of Illumina is a powerful tool for whole-genome gene expression analysis. Expression BeadChips for different organisms like human and mouse are available covering up to 47,000 transcripts and splice variants per sample. Sample requirements for the direct hybridization assay are outlined under Project Design.