Label-free quantitative imaging of living cells and tissues

 Technology & Features

  Digital holographic microscopy (DHM) provides label-free quantitative  phase imaging of biological specimens
  for modular integration into common optical microscopes. The reconstruction of digitally captured holograms
  is performed numerically. This feature allows multi-focus imaging of specimen parts in different layers and
  subsequent autofocussing from single captured digital holograms without mechanical focus realignment. DHM
  requires only low light intensities for object illumination and thus minimizes the interaction with the sample. This
  is an important advantage for minimally invasive quantitative long-term monitoring of living cell cultures. DHM
  phase contrast imaging simplifies automated object tracking and image segmentation for quantification of cell
  migration and morphology by extraction of absolute biophysical parameters such as cellular volume, thickness
  and dry mass. The evaluation of quantitative phase images also provides access to the cellular refractive index
  which is related to the cellular water content, intracellular solute concentrations and tissue density.

  Biomedical Applications

  DHM allows multimodal quantitative imaging of living cells and unstained  tissue sections and can be utilized to
  investigate the influence of drugs, toxins and nanoparticles on cell morphology, growth and motility as well as for
  quantification of inflammation mediated tissue density changes. Moreover it enables the analysis of the cellular
  response to optical manipulation, the quantification of wound healing in-vitro and the observation of cancer cell
  migration in 3D tissue models.